Quick answer
Epitalon (also spelled Epithalon) is a synthetic four-amino-acid peptide, Ala-Glu-Asp-Gly (AEDG), with CAS number 307297-39-8 and a molecular weight of 390.35 g/mol. It is studied in cell-culture and animal research on telomerase, chromatin and pineal signaling. A 10mg research vial holds 10mg of lyophilized powder; its certificate of analysis (COA) should show the lot number, HPLC purity and a mass-spec identity check that matches AEDG.
Epithalon 10mg · lot-specific COA included · HPLC purity and mass-spec identity tested by an independent lab
Table of Contents
- What Is Epitalon (Epithalon)?
- Epitalon Key Facts
- Epitalon Peptide Sequence, CAS Number and Molecular Weight
- Epitalon COA: How to Read the Certificate of Analysis
- Epitalon HPLC Purity and Mass Spec Identity
- How to Reconstitute Epitalon 10mg for Lab Work
- Epitalon Storage Guidelines and Shelf Life
- Epitalon vs Epithalamin vs N-Acetyl Epitalon Amidate
- What Published Epitalon Studies Examined
- Regulatory Status
- Where to Buy Epithalon 10mg
- Frequently Asked Questions
What Is Epitalon (Epithalon)?
Epitalon is a synthetic tetrapeptide made of four amino acids in a fixed order: alanine, glutamic acid, aspartic acid and glycine (Ala-Glu-Asp-Gly, or AEDG). Epitalon, Epithalon and Epithalone are all names for the same compound.
It was designed from the amino acid composition of epithalamin, a polypeptide extract of bovine pineal gland, and has been studied for about 25 years in cell-culture, animal and computer-modelling work (Araj et al., 2025 review). Most of that research looks at telomerase, chromatin structure and pineal signaling.
CoreVionRX supplies Epithalon as a 10mg lyophilized research vial for laboratory use only.
Epitalon Key Facts
- Sequence: Ala-Glu-Asp-Gly (AEDG), 4 amino acids
- Molecular formula: C14H22N4O9
- Molecular weight: 390.35 g/mol (PubChem CID 219042)
- CAS number: 307297-39-8
- Vial contents: 10mg net peptide, lyophilized (freeze-dried) powder
- Testing: each lot is checked by an independent lab for HPLC purity and mass-spec identity; the lot COA ships with the order
- Storage: sealed powder at -20°C away from light; after dissolving, 2–8°C
- Status: research use only; not approved by the FDA for any use
Epitalon Peptide Sequence, CAS Number and Molecular Weight
The Epitalon peptide sequence is Ala-Glu-Asp-Gly, read from the N-terminus (alanine) to the C-terminus (glycine). Its CAS number is 307297-39-8, its molecular formula is C14H22N4O9 and its molecular weight is 390.35 g/mol.
| Property | Value |
|---|---|
| Sequence | Ala-Glu-Asp-Gly (AEDG) |
| Molecular formula | C14H22N4O9 |
| Molecular weight | 390.35 g/mol |
| Monoisotopic mass | 390.14 Da |
| CAS number | 307297-39-8 |
| PubChem CID | 219042 |
| Net peptide per vial | 10mg |
| Form | Lyophilized powder |
Two of the four residues, glutamic acid and aspartic acid, carry acidic side chains, so the molecule has three carboxylic acid groups in total (two side chains plus the C-terminus). Source for formula, weight and CAS: PubChem CID 219042.
Epitalon COA: How to Read the Certificate of Analysis
An Epitalon COA is the lab report that ties one production lot to its test data. It is only useful if it is lot-specific, names its test methods and shows identity as well as purity.
The graphic above is an example layout with placeholder values, not a real lot report. Check these five points on any Epitalon certificate of analysis:
- Lot number: it must match the number printed on your vial label. If it doesn’t, the report doesn’t describe your material.
- Named method: purity should come from HPLC (high-performance liquid chromatography), and the report should say so.
- Purity with a chromatogram: a percentage backed by the trace it came from, with one dominant peak.
- Identity by mass spectrometry: the measured mass should fit Epitalon (390.35 g/mol), not a modified analog.
- Traceable sign-off: the testing lab’s name, the report date and an authorizing signature.
Every CoreVionRX Epithalon lot is tested by an independent lab before it reaches the shelf, and the lot-specific COA comes with your order. For a broader walkthrough, see the peptide endotoxin and COA testing guide.
Epitalon HPLC Purity and Mass Spec Identity
HPLC answers “how much of this is the target peptide?” and mass spectrometry answers “is the target peptide the right one?” An Epitalon test report needs both.
In an HPLC chromatogram, purity is the main peak’s share of the total peak area. Small extra peaks are impurities such as truncated sequences or synthesis by-products. A purity figure with no chromatogram, no method and no lot number tells you very little.
Mass spectrometry confirms identity. Epitalon’s monoisotopic mass is 390.14 Da, so in positive-ion mode the main signal ([M+H]+) appears near m/z 391.1. A clearly different mass suggests a different compound, such as an acetylated or amidated analog.
| Test | What it confirms | What to look for on the report |
|---|---|---|
| HPLC | Purity | Method stated, percentage, chromatogram with one dominant peak |
| Mass spectrometry | Identity | Mass consistent with 390.35 g/mol ([M+H]+ near 391.1) |
| Lot traceability | That the data describes your vial | Lot number that matches the vial label, test date, lab name |
How to Reconstitute Epitalon 10mg for Lab Work
To reconstitute Epitalon, add bacteriostatic water slowly down the inside wall of the vial and swirl gently until the powder dissolves. The volume you add only sets the concentration; the 10mg of peptide in the vial stays the same.
Lab prep steps:
- Let the sealed vial reach room temperature before opening, so moisture doesn’t condense on the powder.
- Wipe the stopper with an alcohol swab.
- Add the chosen volume of bacteriostatic water slowly down the vial wall, not straight onto the powder.
- Swirl gently. Don’t shake, which can cause foaming.
- Check that the solution is clear and colorless, then label the vial with lot number, date, volume added and concentration.
How Much Bacteriostatic Water for Epitalon 10mg?
There is no single required volume. Divide 10mg by the millilitres added to get the concentration in mg/mL:
| Bacteriostatic water added | Epitalon in vial | Resulting concentration |
|---|---|---|
| 1 mL | 10mg | 10 mg/mL |
| 2 mL | 10mg | 5 mg/mL |
| 3 mL | 10mg | 3.33 mg/mL |
| 5 mL | 10mg | 2 mg/mL |
Larger volumes give lower concentrations. If several people in a lab prepare solutions, agree on one standard volume and record it the same way every time. The complete reconstitution guide covers technique in more detail.
Epitalon Storage Guidelines and Shelf Life
Store sealed Epitalon powder frozen at -20°C, away from light and moisture. Once dissolved, keep it refrigerated at 2–8°C and plan on about one week as the conservative window.
| State | Temperature | Typical window |
|---|---|---|
| Sealed powder, long term | -20°C (freezer) | Months, often longer |
| Sealed powder, short term | Room temperature (20–25°C) | Days to a few weeks, such as during shipping |
| Dissolved solution | 2–8°C (refrigerator) | About one week |
| Dissolved, longer | -20°C, in single-use aliquots | Avoid repeated freezing and thawing |
Appearance: Epitalon is a white lyophilized powder that dissolves to a clear, colorless solution. Cloudiness, visible particles or a yellow tint mean the vial should be set aside and checked against its lot COA. The full peptide storage guide explains why these windows apply.
Epitalon vs Epithalamin vs N-Acetyl Epitalon Amidate
These three names describe different materials. Epitalon is the defined four-amino-acid peptide; epithalamin is a pineal gland extract; N-acetyl Epitalon amidate is a chemically modified form of Epitalon.
| Epitalon (AEDG) | Epithalamin | N-Acetyl Epitalon Amidate | |
|---|---|---|---|
| What it is | Synthetic tetrapeptide | Polypeptide extract of bovine pineal gland | Epitalon with an acetyl group on the N-terminus and an amide on the C-terminus |
| Composition | One defined sequence, Ala-Glu-Asp-Gly | A mixture of peptides | Ac-Ala-Glu-Asp-Gly-NH2 |
| Molecular weight | 390.35 g/mol | Not a single molecule | About 431.4 g/mol (calculated) |
| On a COA | Mass near 390 g/mol | Not applicable | Mass near 431 g/mol |
Because the modified form has a different mass, the mass-spec section of a COA tells you which one is in the vial. CoreVionRX’s Epithalon 10mg is the unmodified Ala-Glu-Asp-Gly peptide.
What Published Epitalon Studies Examined
Most Epitalon research is preclinical: cell cultures, animal models and molecular modelling. Much of the early work came from one research group in St. Petersburg, and independent replication is still limited.
- Telomerase in human fibroblasts (cell culture). Adding Epitalon to telomerase-negative human fetal fibroblast cultures was reported to induce the telomerase catalytic subunit, telomerase activity and telomere elongation (Khavinson et al., 2003, Bull Exp Biol Med).
- Telomere length in normal and cancer cell lines (cell culture). An independent UK group exposed two breast cancer cell lines and normal epithelial and fibroblast cells to Epitalon. Normal cells showed telomere lengthening through hTERT and telomerase upregulation, while the cancer lines showed lengthening through the ALT pathway (Al-Dulaimi et al., 2025, Biogerontology).
- Chromatin in cultured lymphocytes (cell culture). Researchers studied ribosomal gene activity and heterochromatin in lymphocytes from donors aged 76–80 and reported heterochromatin decondensation after Epitalon exposure (Khavinson et al., 2003, Neuro Endocrinol Lett).
- Gene expression in stem cells (cell culture and modelling). In human gingival mesenchymal stem cells, AEDG raised mRNA of four neuronal differentiation markers 1.6–1.8 fold; molecular modelling suggested binding to histones H1/3 and H1/6 (Khavinson et al., 2020, Molecules).
- Aging biomarkers in mice (animal). A study of female SHR mice (54 per group) measured biomarkers of aging, life span and spontaneous tumor incidence. It found no change in mean life span, body weight or food intake, and differences in several secondary measures (Anisimov et al., 2003, Biogerontology).
- Review of the field. A 2025 review summarizes in vitro, in vivo and in silico work and notes that physico-chemical and structural studies of Epitalon remain limited (Araj et al., 2025, Int J Mol Sci).
What is still unknown: how cell and animal findings relate to any other setting, and which mechanisms explain them. The 2025 review states that it remains uncertain whether the reported mechanisms are the only ones involved.
Regulatory Status
Epitalon is not approved by the U.S. Food and Drug Administration for any use. CoreVionRX sells it strictly as a laboratory research material. It is not for human or veterinary use and is not sold as a drug, supplement or medical product.
Where to Buy Epithalon 10mg
Epithalon 10mg is available from CoreVionRX with independent HPLC purity and mass-spec identity testing and a lot-specific COA included with every order. See the Epithalon 10mg product page for current price and availability.
Epithalon 10mg · lot-specific COA included · ships with independent test data
Researchers working on related compounds may also want the NAD+ 500mg, MOTS-c 40mg and Semax 10mg research compounds.
Frequently Asked Questions
What is Epitalon?
Epitalon (Epithalon) is a synthetic tetrapeptide with the sequence Ala-Glu-Asp-Gly (AEDG). It was designed from the composition of the pineal extract epithalamin and is studied in cell-culture and animal research on telomerase and chromatin.
What is the Epitalon peptide sequence and CAS number?
The sequence is Ala-Glu-Asp-Gly, written AEDG in one-letter code: alanine, glutamic acid, aspartic acid, glycine. The CAS number is 307297-39-8, the molecular formula is C14H22N4O9 and the molecular weight is 390.35 g/mol.
What should an Epitalon certificate of analysis show?
A lot number that matches your vial, HPLC purity with the method and chromatogram, mass-spec identity consistent with 390.35 g/mol, and the testing lab’s name, report date and sign-off.
How much bacteriostatic water do you add to Epitalon 10mg?
It depends on the concentration you want. 1 mL gives 10 mg/mL, 2 mL gives 5 mg/mL, 3 mL gives 3.33 mg/mL and 5 mL gives 2 mg/mL. The volume changes only the concentration, not the amount of peptide.
How should Epitalon be stored?
Keep the sealed powder frozen at -20°C, away from light and moisture. After dissolving, refrigerate at 2–8°C and plan on about one week; for longer, freeze single-use aliquots at -20°C.
What is the difference between Epitalon and Epithalon?
None. Epitalon, Epithalon and Epithalone are spellings of the same AEDG peptide. N-acetyl Epitalon amidate is a different, modified compound.
Related Research Guides
Written by the CoreVionRX Research Team. Sources: PubChem CID 219042 and the PubMed records linked above.
For laboratory research use only. Not for human or veterinary use.
